Copper Acetate Manufacturers(WSDTY) proposed Metal shocks, RNA isolation, and quantitative reverse transcription-PCR (qRT-PCR).R. Copper Acetate palustris cultures were grown anaerobically as described above and were placed in an anoxic chamber for shocks. In order to obtain enough cell mass for RNA isolation, we performed shocks on mid-log-phase cultures. Shocks were performed with 1 mM Fe(II) and/or 1 μM Cu(II). These concentrations were chosen to avoid significant copper contamination in the Fe(II) shocks while maintaining metal concentrations high enough to ensure a transcriptional response. After 15 min, 6 ml of culture was added to an equal volume of RNALater (Ambion), and the mixture was centrifuged for 6 min at 6,000g at room temperature. The supernatant was poured off, and the pellets were frozen in liquid nitrogen and were stored until RNA extraction.
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