Copper Acetate Manufacturers(WSDTY) proposed pheochromocytoma cells originating in rat adrenal medulla, Copper Acetate, were obtained from RIKEN Cell Bank. The PC12 cells were grown and maintained in DMEM supplemented with 10% fetal bovine serum, 10% horse serum, 100?U/mL penicillin , and 100?μg/mL streptomycin (Invitrogen) at 37°C under 5% CO2 atmosphere. The cells were passaged before reaching confluence using 0.025% (v/v) trypsin/EDTA in PBS. For the differentiation of PC12 cells, the cells were seeded on a collagen-coated dish at a density of 2.0×104 cells/cm2. Then, the cells were exposed to 50ng/mL NGF in medium containing 1% HS for 24h after seeding in the maintenance medium. The medium was exchanged every two days with fresh DMEM containing 50ng/mL NGF and 1% HS until six days after the induction of differentiation. The cells cultured in the differentiation medium containing 1% HS without the NGF treatment served as control and are called naive cells in this study.
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